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KMID : 0438219730100030797
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Korea University Medical Journal
1973 Volume.10 No. 3 p.797 ~ p.805
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A Study on Phytohemoagglutinin in Lenzites betulina L. Native to Korea
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Abstract
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The author studied 23 kinds of saline extract of mushrooms native to Korea on its agglutinating activity with human RBC. Among them, Lenzites betulina L. (LBL) was found to have phytohemoagglutinin activity and studied on its agglutination titer, absorption test with human RBC, agglutination inhibition test with human saliva and various sugars, heat stability, salting out test, sperm agglutination test and triple bond reaction.
The results could be summarized as follows:
1. Anti B-lectin was found in LBL
2. Anti B-lectin in LBL was absorbed by same group´s RBC and saliva of secretors.
3. The agglutinating titers of anti B-lectin in LBL were 1:32 with group B RBC and 1:16 with group AB RBC.
4. The anti B-lectin was contained in precipitates of LBL treated by ammonium sulfate in concentration of 50% saturation.
5. The agglutination activity of anti LBL was inhibited by L-fucose, D-fucose, D-galactose, lactose and D-melibiose.
6. The human sperms were not agglutinated by PHA in LBL.
7. It was possible to detect blood type of "B" group in the blood stains and various humoral stains by triple bond test with anti-B lectin in LBL.
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